Uttam SURANA    
                       
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  Uttam SURANA  
  Lab Location: #6-12

email:
mcbucs@imcb.a-star.edu.sg
tel:65869503
 
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  Key Publications  
 


Chen C, Lim HH, Shi J, Tamura S, Maeshima K, Surana U and Gan L (2016)
Budding yeast chromatin is dispersed in a crowded nucleoplasm.
Mol Biol Cell 27:3357-3368

Zhang T, Si-Hoe SL, Hudson DF and Surana U (2016)
Condensin recruitment to chromatin is inhibited by checkpoint kinase Chk2 in response to DNA damage
Cell Cycle (In press)

Liang H, Esposito A, De S, Ber S, Collin P, Surana U, Venkitaraman AR . (2014)
Homeostatic control of polo-like kinase-1 engenders non-genetic heterogeneity in G2 checkpoint fidelity and timing
Nature Commun 5:4048.

Yu H, Lim HH et al. (2014)
Chaperoning HMGA2 protein protects stalled replication forks in stem and cancer cells
Cell Rep 6:684-697

Liang H., Lim H.H., Venkitaraman A. and Surana U. (2012)
Cdk1 promotes kinetochore bi-orientation and regulates Cdc20 expression during recovery from spindle checkpoint arrest
EMBO J 31:403-416.

Phong M.S., Van-Horn R.D., Li S., Tucker-Kellog G., Surana U. and Ye X.S. (2010)
The p38 MAPK promotes cell survival but is not required for G2 checkpoint arrest of cancer cells in response to DNA damage
Mol. Cell. Biol. 30:3816-3826

Zhang T., Nirantar S., Lim H. H., Sinha I., and Surana U. (2009)
DNA damage checkpoint maintains Cdh1 in active state to inhibit anaphase progression
Developmental Cell, 17:541-551.

Lim H. H., Zhang T. and Surana U (2009)
Regulation of Centrosome Separation in yeast and vertebrates: common threads
Trends in Cell Biology, 19:325-333.

Crasta, K., Lim, H. H., Giddings Jr, T. H., Winey, M. and Surana, U. (2008)
Inactivation of Cdh1 by synergistic action of Cdk1 and Polo kinase is necessary for proper assembly of mitotic spindle.
Nature Cell Biology10:665-675.

Krishnan, V., Dirick, L., Lim, H. H., Lim, T. S. J., Si-Hoe, S. L., Cheng, C. S., Yap, K., Ting, A., Schwob, E. and Surana, U. (2007) A small molecule inhibitor stalls replication forks and activates S phase checkpoint.
Cell Cycle 6:13, 1621-1630.

Crasta, K., Huang, P., Morgan, G., Winey, M. and Surana, U. (2006) Cdk1 regulates centrosome separation by restraining proteolysis of microtubule-associated proteins.
EMBO J. 25:2551-2563.

Zhang, T., Lim, H. H., Cheng, C. S. and Surana, U. (2006)
Deficiency of centromere-associated protein Slk19 causes premature nuclear migration and loss of centromeric elasticity.
J. Cell Science 119:519-531.

Krishnan, V., Nirantar, S., Crasta K., Cheng, A. Y. H. and Surana, U. (2004)
DNA-Replication Checkpoint prevents precocious chromosome segregation by regulating spindle dynamics.
Mol. Cell 16:687-700.

Lim, H. H., Yeong, F. M. and Surana, U. (2003)
Inactivation of mitotic kinase triggers translocation of MEN components to mother daughter neck in yeast.
Mol. Biol Cell 14:4734-4743.

Lim, H. H. and Surana, U. (2003) Tome-1, wee1 and onset of mitosis: coupled destruction for timely entry
Mol. Cell 11:845-846.

 
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    Uttam SURANA
 


Uttam Surana undertook his graduate studies at the University of Arizona and obtained a PhD from the Dept. of Molecular and Cellular Biology in 1986. Thereafter, he moved to the Dept. of Engineering at the University of Cambridge where he spent 2 years studying the mechanical properties of bacterial cell surface polymers and their role in cell shape determination. He spent the subsequent four years as a postdoctoral fellow at the Institute of Molecular Pathology in Vienna investigating various aspects of cell division in the budding yeast. He joined the IMCB in 1992. For his outstanding contribution to the understanding of control circuits that regulate cell cycle, Dr Surana was awarded in 2007 Singapore’s National Science Award.

       
    Regulation of the Cell Division Cycle
   


Cellular activities leading to division are highly regulated both in time and space. Violation of this precision can lead to genomic instability which may result in reduced cellular fitness, unbridled proliferation (cancerous growth) or death of parent and progeny cells. Valuable insights into the way cell division is controlled in complex systems, such as human cells, have come from investigations of relatively simple eukaryotes. The budding yeast Saccharomyces cerevisiae, (the model system we use for our investigations) is one such organism that has served remarkably well due to its amenability to genetic manipulations and because many of the pathways that regulate cell division are highly conserved from yeast to man.

Coordinated execution of cell cycle events is, in-part, imposed by checkpoint controls that ensure that a given event is not initiated until the preceding event is successfully completed. During S phase the genome is monitored by two such major control pathways, namely, DNA replication checkpoint and DNA damage checkpoint. When cells incur replication stress or DNA damage, these pathways prevent initiation of chromosome segregation until the damage is repaired. We have focused our investigations on the mechanisms by which checkpoint pathways prevent execution of mitotic events. Using various genetic and biochemical analysis, we have discovered that the replication checkpoint prevents premature chromosome segregation by directly regulating spindle dynamics, thereby inhibiting precocious spindle elongation. This has led us to closely examine the process of spindle assembly itself. We have uncovered a novel mechanism by which duplicated centrosomes are separated and moved apart to assemble a bipolar spindle. Our findings suggest that cyclin dependent kinase (Cdk1) and polo kinase act synergistically (with Cdk1 serving as a priming kinase for polo kinase) to inactivate the ubiquitin ligase APCCdh1 that mediates the proteolytic destruction of Cin8 and Kip1 kinesins essential for centrosome separation. An extension of these studies has revealed that the regulatory circuit involving Cdk1, Polo kinase, Cdh1 and kinesins is also utilized by the DNA damage checkpoint to prevent segregation of damaged chromosomes. Hence mitotic spindle has emerged as a novel target of S phase checkpoints. To take these studies forward we are investigating the mechanism by which hyper-activation of Rad53 (Chk2) during G2/M leads to mitotic spindle collapse

Recovery from (resumption of cell cycle progression after repair) or adaptation to (escape from arrest in the absence of repair) checkpoint-imposed arrest are also critical aspects of checkpoint regulation and have important implications for chromosome stability. Efforts are now underway to delineate the ‘adaptation pathway’ utilized by cells to escape from DNA-damage-induced arrest

By studying the coordination of various cellular events, we hope to understand the molecular circuits through which eukaryotic cells exercise temporal and spatial control over their activities during cell division. In addition to providing clues to the organizing principles of living cells, such efforts may also lead to the identification of key regulator that could serve as targets for the therapeutic interventions against growth of cancerous cells. We are currently conducting screens to identify anti-proliferative compounds targeting key cell cycle regulators.