Vladimir KORZH   
                       
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  Vladimir KORZH  
  Lab Location: #8-13

email:
vlad@imcb.a-star.edu.sg
tel:65869748
 
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  Key Publications  
 

Le, M.T., Teh, C., Shyh-Chang, N., Xie, H., Zhou, B., Korzh, V., Lodish, H.F., and Lim, B. (2009) . MicroRNA-125b is a novel negative regulator of p53.
Genes Dev, 23 (7):862-876. 

Vasilyev A, Liu Y, Mudumana S, Mangos S, Lam P, Majumdar A, Zhao J, Poon KL, Kondrychyn I, Korzh V, Drummond I. (2009)
Collective Cell Migration Drives Morphogenesis of the Kidney Nephron.
PLoS Biol , 7(1): e9

García-Lecea M, Kondrychyn I, Fong SH, Ye Z-R, Korzh V (2008)
In vivo Analysis of Choroid Plexus Morphogenesis in Zebrafish.
PLoS ONE
3(9): e3090

Fong, S., Emelyanov A., Teh C. and Korzh V. (2005) Wnt signaling mediated by Tbx2b regulates cell migration during formation of the neural plate. Development 132(16):3587-3596.

Parinov, S., Kondrichin, I., Korzh, V. and Emelyanov, A. (2004)
Enhancer trap insertional mutagenesis as a tool for identification of developmentally regulated genes in zebrafish.
Dev. Dynam, 231, 449-459.

Teh, C., Chong, S.-W. and Korzh, V. (2003)
DNA delivery into neural tube of zebrafish embryo by electroporation. BioTechniques 35:950-954.

Dheen, S. T., Sleptsova-Friedrich, I., Xu, Y., Clark, M., Gong, Z., Lehrach, H., Korzh, V. (1999)
Zebrafish tbx-c functions during formation of midline structures.
Development 126, 2703-2713.

Sampath, K., Rubinstein, A., Cheng, A., Liang, J., Fekany, K., Solnica-Krezel, S., Korzh, V., Halpern, M., Wright, C.V.E. (1998).
Induction of the zebrafish ventral brain and floorplate requires cyclops/nodal signalling.
Nature 395, 185-189.

 
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  Vladimir KORZH


After graduating from Kiev University in 1976, Vladimir Korzh got his PhD in developmental biology in 1981 at the Koltsov Institute of Developmental Biology in Moscow. He worked as a researcher in the same institute until 1990 when he moved as a postdoc to the University of Tromsø, Norway and started using zebrafish as a model of vertebrate neurodevelopment. Later, he moved to the University of Umeä, Sweden and in 1995 started a laboratory of fish developmental biology at the ex-Institute of Molecular Agrobiology in Singapore before joining the IMCB in 2002. He is an Associate Professor.

     
  Zebrafish Translational Unit
 


The Zebrafish Translational Unit (ZTU) aim is to develop novel in vivo test systems and approaches that address efficacy of novel drug delivery platforms, drugs screening and testing, and validation of their biocompatibility. The ZTU personnel has experience in developing transgenics and mutants of specific genes as well as analysis activity of signaling pathways during development of multiple systems of organs, in particular, the central nervous system and cardiovascular system. Exposure of a panel of transgenic embryos to a specific chemical may help to address its potential toxicity and/or effect on development of most, if not all, tissues.

Researchers of the ZTU have generated and/or maintain a number of transgenic lines with expression of different fluorescent proteins as cytosolic, membrane and nuclear markers in different cell lineages and tissues, including, but not limited to the heart (endocardium, myocardium, pericardium, conduction system, valves, etc), blood vessels, CNS (brain, ventricular system, spinal cord), PNS (sensory ganglia, lateral line, mechanoreceptors, support and mantle cells), swimbladder, liver, pancreas, epithelium, limbs, intestine, kidney, immune system, etc. These were used to establish several in vivo models including but not limited to some forms of cancer, oxidative stress, hemorrhagic stroke, and so on. In addition to reporter strains, multiple mutant lines with developmental defects in various cell lineages and/or organs, including cilia, immune cells, etc, were developed too. This opens a possibility for embryos with reduced gene doses to be used in drug screens as sensitized backgrounds or in screens for compounds that attenuate the severity of the respective phenotypes.

Recently the ZETRAP database containing information about transgenic ET lines has been developed in  the lab. Secondly, a novel technique of massive electroporation of DNA into  a neural tube of a zebrafish embryo has solved a long-standing problem in analyzing of  gene function during late neurodevelopment in the zebrafish. For a detailed protocol click here.