Central Imaging Facility
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1. Transparent 3D confocal image rotation of mouse kidney section

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CIF's Video 1

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Sample: mouse kidney section (FluoCells prepared slide #3 by Life Technologies)
Blue: DAPI staining of nucleus
Green: elements of glomeruli and convoluted tubules labeled with green-fluorescent Alexa Fluor® 488 wheat germ agglutinin
Red: F-actin prevalent in glomeruli and brush border stained with red-fluorescent Alexa Fluor® 568 phalloidin
Acquisition: Plan-Apochromat 63x/1.40 Oil DIC M27 objective on Zeiss LSM700 Confocal microscope, imaged by Samuel Ko from Zeiss.
Post-acquisition: 3D reconstruction of z-stack images with Zen software, 180o rotation along Y-axis.

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