Sample: fixed, permeabilized Muntjac skin fibroblast cells (FluoCells prepared slide #6 by Life Technologies)
Green: F-actin labeled with green-fluorescent Alexa Fluor® 488 phalloidin
Red: Mitochondria labeled with mouse anti–OxPhos Complex V inhibitor protein antibody and visualized using orange-fluorescent Alexa Fluor® 555 goat anti–mouse IgG antibody
Acquisition: EC Plan-Neofluar 20x/0.50 M27 objective on Zeiss LSM700 Confocal microscope, imaged by Melvin Sin from CIF.
Post-acquisition: maximum intensity projection of z-stack images with Zen software.
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