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Bioimage Acquisition

Image 6
Sample: mouse kidney section (FluoCells prepared slide #3 by Life Technologies)
Blue: DAPI staining of nucleus
Green: elements of glomeruli and convoluted tubules labeled with green-fluorescent Alexa Fluor® 488 wheat germ agglutinin
Red: F-actin prevalent in glomeruli and brush border stained with red-fluorescent Alexa Fluor® 568 phalloidin
Grey: Differential Interference Contrast (Nomarski microscopy)
Acquisition: Plan Apochromat 20x/0.80 Ph2 M27 objective on Zeiss AxioImager.Z1 microscope, imaged by Melvin Sin from CIF.

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