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Bioimage Acquisition

Image 4
Sample: dorsal view of live 3 days-post-fertilization ET16 transgenic zebrafish embryo (refer to ZETRAP)
Green: enhanced Green Fluorescent Protein
Acquisition: Plan-Neofluar 10x/0.3 objective, 0.5x digital zoom on Zeiss LSM700 Confocal microscope, imaged by Melvin Sin from CIF.
Post-acquisition: maximum intensity projection of z-stack images with Zen software, removal of background noise with ImageJ.

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