Frederic BARD  
                       
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  Frederic BARD  
  Lab Location: #5-12

email:
fbard@imcb.a-star.edu.sg
tel:65869585
 
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  Key Publications  
 

Bard F, Chia J.
Cracking the Glycome Encoder: Signaling, Trafficking, and Glycosylation.
Trends Cell Biol. 2016 May;26(5):379-88. Review.

Yang BX, El Farran CA, Guo HC, Yu T, Fang HT, Wang HF, Schlesinger S, Seah YF, Goh GY, Neo SP, Li Y, Lorincz MC, Tergaonkar V, Lim TM, Chen L, Gunaratne J, Collins JJ, Goff SP, Daley GQ, Li H, Bard FA, Loh YH.
Systematic identification of factors for provirus silencing in embryonic stem cells.
Cell. 2015 Sep 24;163(1):230-45.

Chaumet A, Wright GD, Seet SH, Tham KM, Gounko NV, Bard F.
Nuclear envelope-associated endosomes deliver surface proteins to the nucleus.
Nat Commun. 2015 Sep 10;6:8218.

Gonzales KA, Liang H, Lim YS, Chan YS, Yeo JC, Tan CP, Gao B, Le B, Tan ZY, Low KY, Liou YC, Bard F, Ng HH.
Deterministic Restriction on Pluripotent State Dissolution by Cell-Cycle Pathways.
Cell. 2015 Jul 30;162(3):564-79.

Chia J, Tham KM, Gill DJ, Bard-Chapeau EA, Bard FA.
ERK8 is a negative regulator of O-GalNAc glycosylation and cell migration.
Elife. 2014 Mar 11;3:e01828.

Gill DJ, Tham KM, Chia J, Wang SC, Steentoft C, Clausen H, Bard-Chapeau EA, Bard FA.
Initiation of GalNAc-type O-glycosylation in the endoplasmic reticulum promotes cancer cell invasiveness.
Proc Natl Acad Sci U S A. 2013 Aug 20;110(34):E3152-61.

Chia J, Goh G, Racine V, Ng S, Kumar P, Bard F.  
RNAi screening reveals a large signaling network controlling the Golgi apparatus in human cells.
Mol Syst Biol. 2012;8:629.

Moreau D, Kumar P, Wang SC, Chaumet A, Chew SY, Chevalley H, Bard F.
Genome-wide RNAi screens identify genes required for Ricin and PE intoxications.
Dev Cell. 2011 Aug 16;21(2):231-44.

Chia NY, Chan YS, Feng B, Lu X, Orlov YL, Moreau D, Kumar P, Yang L, Jiang J, Lau MS, Huss M, Soh BS, Kraus P, Li P, Lufkin T, Lim B, Clarke ND, Bard F*, Ng HH*. (*corresponding authors)
A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity.
Nature. 2010 Nov 11;468(7321):316-20. 

Gill DJ, Chia J, Senewiratne J, Bard F.
Regulation of O-glycosylation through Golgi-to-ER relocation of initiation enzymes.
J Cell Biol.2010 May 31;189(5):843-58.

Bard F, Casano L, Mallabiabarrena A, Wallace E, Saito K, Kitayama H, Guizzinti G, Hu Y, DasGupta R, Perrimon N, Malhotra V. Functional genomics reveals new genes involved in protein secretion and Golgi organization.
Nature. 2006 Feb 2;439(7076):604-7

 
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  Frederic BARD


Frederic Bard did his graduate work at Yale University, USA and at the Ecole Normale Superieure of Lyon, France where he obtained his PhD. He worked on the dynamics of the sealing zone, a unique actin cytoskeleton structure in osteoclasts required for bone resorption. During his postdoctoral work at the University of California San Diego (2001-2006), he identified a collection of new genes essential for general protein secretion, the TANGO genes. After starting his group IMCB in 2006, he has established a genome-wide RNAi screening platform. The group has focused on membrane trafficking regulation, Golgi organisation and how signalling at the Golgi can control protein glycosylation.

     
  Regulation of Membrane Traffic
 


The human cell is organized into multiple intracellular compartments. Compartmentalization controls many aspects of cellular physiology and has increased in complexity throughout evolution. Most cellular compartments are bound, like the whole cell itself, by lipid-based membranes and exchange material through the trafficking of membrane-bound structures. We wish to understand how this membrane traffic is regulated to mediate various cellular functions. One of the technologies we use to address these questions is RNA interference screening at the genomic scale, which allows us to identify novel key players in these processes.

We focus on two questions:

1) How trafficking regulation at the Golgi complex affects glycosylation in health and disease
2) How intracellular trafficking is exploited by pathogens and toxins

The Golgi apparatus

    Legend: Human Hela cells stained for the nucleus (blue), the microtubules network (red) and the Golgi apparatus (green).