Frederic BARD  
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  Frederic BARD  
  Lab Location: #5-12

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  Key Publications  

Chia J, Tham KM, Gill DJ, Bard-Chapeau EA, Bard FA.
ERK8 is a negative regulator of O-GalNAc glycosylation and cell migration.
eLife. 2014;3:e01828.

Gill DJ, Tham KM, Chia J, Chyii WS, Steentoft C, Clausen H, et al.
Initiation of GalNAc-type O-glycosylation in the endoplasmic reticulum promotes cancer cell invasiveness.
Proceedings of the National Academy of Sciences. 2013 Aug 2.

Chia, J., Goh, G., Racine, V., Ng, S., Kumar, P., & Bard, F.
RNAi screening reveals a large signaling network controlling the Golgi apparatus in human cells.
Molecular Systems Biology (2012) 8, 629. doi:10.1038/msb.2012.59

Dimitri Moreau, Pankaj Kumar, Shyi Chyi Wang, Alexandre Chaumet, Shin Yi Chew, Hélène Chevalley, Frédéric Bard.
Genome-Wide RNAi Screens Identify Genes Required for Ricin and PE Intoxications.
Dev Cell (2011) vol. 21 (2) pp. 231-44.

Gill D, Clausen H, Bard F
Location, Location, Location: New insights into O-GalNAc protein glycosylation.
Trends in Cell Biology. 2010 Dec 8; Epub ahead of print.

Chia NY, Chan YS, Feng B, Lu X, Orlov YL, Moreau D, Kumar P,Yang L, Jiang J, Lau MS, Huss M, Soh BS, Kraus P, Li P, Lufkin T, Lim B, Clarke N, Bard F*, Huck-Hui Ng* (* corresponding authors)
A genome-wide RNAi screen reveals determinants of human ES cell identity.
2010 Nov 11;468(7321):316-20.

Gill DJ, Chia J, Senewiratne J, Bard F.
Regulation of O-glycosylation through Golgi-to-ER relocation of initiation enzymes.
J Cell Biol. 2010 May 31;189(5):843-58.

R Bard F, Malhotra V
Transit from the Trans-Golgi Network to the plasma membrane.
Annu Rev Cell Dev Biol.

Bard F, Casano L, Mallabiabarrena A, Wallace E, Saito K, Kitayama H, Guizzinti G, Hu Y, DasGupta R, Perrimon N, Malhotra V. Functional genomics reveals new genes involved in protein secretion and Golgi organization.Nature. 2006 Feb 2;439(7076):604-7

Bard F, Mazelin L, Pechoux-Longin C, Malhotra V, Jurdic P.
Src regulates Golgi structure and KDEL receptor-dependent retrograde transport to the endoplasmic reticulum.             J Biol Chem. 2003 Nov 21;278(47):46601-6.

Destaing O, Saltel F, Geminard JC, Jurdic P, Bard F. Podosomes display actin turnover and dynamic self-organization in osteoclasts expressing actin-green fluorescent protein. Mol Biol Cell. 2003 Feb;14(2):407-16.

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  Frederic BARD

Frederic Bard did his graduate work at Yale University, USA and at the Ecole Normale Superieure of Lyon, France where he obtained his PhD. He worked on the dynamics of the sealing zone, a unique actin cytoskeleton structure in osteoclasts required for bone resorption. During his postdoctoral work at the University of California San Diego (2001-2006), he identified a collection of new genes essential for general protein secretion, the TANGO genes. After starting his group IMCB in 2006, he has established a genome-wide RNAi screening platform. The group has focused on membrane trafficking regulation, Golgi organisation and how signalling at the Golgi can control protein glycosylation.

  Regulation of Membrane Traffic

The human cell is organized into multiple intracellular compartments. Compartmentalization controls many aspects of cellular physiology and has increased in complexity throughout evolution. Most cellular compartments are bound, like the whole cell itself, by lipid-based membranes and exchange material through the trafficking of membrane-bound structures. We wish to understand how this membrane traffic is regulated to mediate various cellular functions. One of the technologies we use to address these questions is RNA interference screening at the genomic scale, which allows us to identify novel key players in these processes.

We focus on two questions:

1) How trafficking regulation at the Golgi complex affects glycosylation in health and disease
2) How intracellular trafficking is exploited by pathogens and toxins

The Golgi apparatus

    Legend: Human Hela cells stained for the nucleus (blue), the microtubules network (red) and the Golgi apparatus (green).