Andrea PAVESI
                       
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  Andrea PAVESI  
  Lab Location: #3-05

email:
andreap@imcb.a-star.edu.sg
tel:65869516
 
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  Key Publications  
 

Ugolini, G. S., PAVESI, A., Rasponi, M., Fiore, G. B., Kamm, R., & Soncini, M. (2017).
Human cardiac fibroblasts adaptive responses to controlled combined mechanical strain and oxygen changes in vitro.
eLife, 6.

Adriani, G., Ma, D., PAVESI, A., Kamm, R. D., & Goh, E. L. K. (2017).
A 3D neurovascular microfluidic model consisting of neurons, astrocytes and cerebral endothelial cells as a blood-brain barrier.
Lab on a Chip, 17(3), 448–459.

Lim, S. H., & PAVESI, A. (2017).
Creating Multiple Organotypic Models on a Single 3D Cell Culture Platform.
BioTechniques, 62(3).

Adriani, G., PAVESI, A., Tan, A. T., Bertoletti, A., Thiery, J. P., & Kamm, R. D. (2016).
Microfluidic models for adoptive cell-mediated cancer immunotherapies.
Drug Discovery Today, 21(9), 1472–1478.

PAVESI, A., Adriani, G., Tay, A., Warkiani, M. E., Yeap, W. H., Wong, S. C., & Kamm, R. D. (2016).
Engineering a 3D microfluidic culture platform for tumor-treating field application.
Scientific Reports, 6(1), 26584.

Tay, A., PAVESI, A., Yazdi, S. R., Lim, C. T., & Warkiani, M. E. (2016).
Advances in microfluidics in combating infectious diseases.
Biotechnology Advances, 34(4), 404–421.

Ugolini, G. S., Rasponi, M., PAVESI, A., Santoro, R., Kamm, R., Fiore, G. B., et al. (2016).
On-chip assessment of human primary cardiac fibroblasts proliferative responses to uniaxial cyclic mechanical strain.
Biotechnology and Bioengineering, 113(4), 859–869.

PAVESI, A., Adriani, G., Rasponi, M., Zervantonakis, I. K., Fiore, G. B., & Kamm, R. D. (2015).
Controlled electromechanical cell stimulation on-a-chip.
Scientific Reports, 5, 11800.

S Koh, CYL Tham, AT Tanoto, A Pavesi, RD Kamm, A Bertoletti.
Engineered HBV-specific T cells: Disentangling antiviral from killing capacity.
Journal of Hepatology, 62, S188 (2015).

Ochs, C. J., Kasuya, J., PAVESI, A., & Kamm, R. D. (2014).
Oxygen levels in thermoplastic microfluidic devices during cell culture.
Lab on a Chip, 14(3), 459–462.

Ochs, C. J., Kasuya, J., PAVESI, A., & Liebsch, G. (2014).
2D-Visualisierung des zellulären Sauerstoff verbrauchs in Mikrofluidiksystemen.
BIOspektrum, 20(7), 773–775.

Uzel, S. G. M., PAVESI, A., & Kamm, R. D. (2014).
Microfabrication and microfluidics for muscle tissue models.
Progress in Biophysics and Molecular Biology, 115(2-3), 279–293.

Pavesi, A., Soncini, M., Zamperone, A., Pietronave, S., Medico, E., Redaelli, A., et al. (2014).
Electrical conditioning of adipose-derived stem cells in a multi-chamber culture platform.
Biotechnology and Bioengineering, 111(7), 1452–1463.

Pietronave, S., Zamperone, A., Oltolina, F., Colangelo, D., Follenzi, A., Novelli, E., et al. (2014).
Monophasic and biphasic electrical stimulation induces a precardiac differentiation in progenitor cells isolated from human heart.
Stem Cells and Development, 23(8), 888–898.

PAVESI, A., Piraino, F., Fiore, G. B., Farino, K. M., Moretti, M., & Rasponi, M. (2011).
How to embed three-dimensional flexible electrodes in microfluidic devices for cell culture applications.
Lab on a Chip, 11(9), 1593–1595.

Vismara, R., PAVESI, A., Votta, E., Taramasso, M., Maisano, F., & Fiore, G. B. (2011).
A pulsatile simulator for the in vitro analysis of the mitral valve with tri-axial papillary muscle displacement.
The International Journal of Artificial Organs, 34(4), 383–391.

 

 

 
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  Andrea PAVESI


Dr. Pavesi was born in Italy, and studied at Politecnico di Milano where he obtained his MS in Biomedical Engineering in 2008. He was awarded with the Interpolytechnic School Fellowship in 2009 and joined the PhD program in Biomedical and Biomechanical Engineering. He spent his last year of the PhD in Boston at the Massachusetts Institute of Technology (MIT), at the Department of Biomedical Engineering, with Prof. Roger Kamm and completed his PhD in 2012. The same year he moved to Singapore to work as Post-doc at The Singapore-MIT Alliance for Research and Technology (SMART) that is a major research enterprise established by the Massachusetts Institute of Technology (MIT) in partnership with the National Research Foundation of Singapore (NRF). In 2014 he became Research Scientist in SMART and in 2017 he joined the Institute of molecular and cell biology (IMCB) in A-Star as Junior Investigator.  His research is now focused on microfluidic models for studying adoptive cell-mediated cancer immunotherapies in tumour microenvironment models.

     
  3D tumour microenvironment in vitro models Lab
 


The group is currently focused on developing and utilizing microfluidic platforms mainly for, but not limited to, cancer research by creating multicellular models with conditions and stimulations that play a fundamental role in specific human tissues.
The powerful microfluidic tool allows the fine tuning of parameters such as: i) co-culture of multiple cell types in 2D and 3D; ii) application of different concentrations and gradients of oxygen; iii) administration of cytokines, chemokines and inflammatory molecules as well as iv) the control of physical parameters, like ECM stiffness and density, to take into consideration the fact that tumour metastasis can seed in different organs or that the TME can be fibrotic and immunosuppressive.

The model represents an efficient preclinical tool to be potentially used during clinical trials to match the optimal drug and technique to individual cancer patients following a personalized medicine strategy.

The Pavesi Lab. in currently focusing on the following topics:

Focus 1: Create a 3D microfluidic model for preclinical evaluation of tumour-specific immunotherapy techniques.
Immunotherapy aims to redirect the patient’s immune system to fight against a specific pathology and holds great promise as novel approach in cancer treatment. Our recently developed microfluidics-based assay was successfully able to mimic the conditions of the patient-specific tumour microenvironment and monitor engineered T cell cytotoxicity on hepatocellular carcinoma associated to hepatitis B virus (HBV+ HCC) cells in a 3D extracellular matrix (ECM)-like environment.

Focus 2: Explore epigenetics chemical probes in a 3D TME model 
Chemical biology methods using selective, cell-active chemical inhibitors constitute a promising approach for drug development. Chemical probes are small molecules that rapidly and selectively inhibit the target protein in cells. One way to facilitate the discovery of new therapeutics against cancer is to use microfluidic technology mimicking the 3D TME conditions to screen these chemical probes.

Focus 3: Investigate myeloid cells interplay in a 3D TME model
TME is responsible of immune suppression by the synergistic effect of several factors. Some of the most crucial mechanisms are: (a) the presence of cancer associated fibroblasts which regulate ECM and the expression of CXC12, (b) the role of B cells and tumour associated macrophages in T cell recruitment, (c) the production of Indole 2,3-dioxygenase (IDO) that compromise T cell proliferation, (d) the oxygen levels which are correlated with PD-L1 expression and cytokines production, (e) the tumour vasculature that preferentially recruits other immune cells compared to T cells and (f) the nitration of chemokine CCL2 resulting in T cell trapping in the stroma.
By creating a TME model, we recapitulate step by step all these elements. Cancer cell aggregates, T cells, macrophages and B cells can be co-cultured in an optimized matrix composed by a mixture of collagen and fibrin containing also endothelial cells and fibroblast in order to create a vasculature network around the tumour mass model.

Contacts:
Andrea Pavesi, PhD - Junior Investigator 
Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A*STAR)
61 Biopolis Drive, #03-05 Proteos Building, Singapore 138673
DID: (65) 6586 9516
Email: andreap@imcb.a-star.edu.sg

Open Positions:
Please contact andreap@imcb.a-star.edu.sg