Ashish K. MAURYA1,2,
Marcel SOUREN1,3, Xingang WANG1, Joachim WITTBRODT3 and Philip W. INGHAM1,2,*.
1 - Institute of Molecular and Cell Biology, 61 Biopolis Drive, Singapore 138673.
2 - Dept of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543.
3 - EMBL Heidelberg, Meyerhofstraße 1, Heidelberg 69117, Germany.
* Author for correspondence (firstname.lastname@example.org)
Published in Development 138: 487-494 (2011).
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Different levels and timing of Hedgehog (Hh) signalling activity have been proposed to specify three distinct cell types in the
zebrafish myotome. Two of these, the medial fast-twitch fibres (MFFs) and the slow-twitch muscle pioneers (MPs) are
characterised by expression of eng1a, -1b and -2a and require the highest levels of Hh for their specification. We have defined a
minimal eng2a element sufficient to drive reporter expression specifically in MPs and MFFs. This element binds both Gli2a, a
mediator of Hh signalling, and activated Smads (pSmads), mediators of bone morphogenic protein (BMP) signalling, in vivo. We
found a strict negative correlation between nuclear accumulation of pSmad, and eng2a expression in myotomal cells and show
that abrogation of pSmad accumulation results in activation of eng2a, even when Hh signalling is attenuated. Conversely, driving
nuclear accumulation of pSmad suppresses the induction of eng expression even when Hh pathway activity is maximal. Nuclear
accumulation of pSmads is depleted by maximal Hh pathway activation. We show that a synthetic form of the Gli2 repressor
interacts with Smad1 specifically in the nuclei of myotomal cells in the developing embryo and that this interaction depends upon
BMP signalling activity. Our results demonstrate that the eng2a promoter integrates repressive and activating signals from the
BMP and Hh pathways, respectively, to limit its expression to MPs and MFFs. We suggest a novel basis for crosstalk between the
Hh and BMP pathways, whereby BMP-mediated repression of Hh target genes is promoted by a direct interaction between Smads
and truncated Glis, an interaction that is abrogated by Hh induced depletion of the latter.