FIGURE 2: Native Baxβ is constitutively in active form and is a potent inducer of Cyto c release from mitochondria
(A) Baxβ is constitutively in an active conformation. MCF-7 cells transfected with the plasmid encoding various GFP-tagged Bax proteins were immunostained for active form of Bax (Act Bax) with the anti-Bax conformation-specific antibody N20 (red).
(B) Baxβ triggers Cyto c in transfected cells. MCF-7 transfected with indicated GFP-Bax expression plasmids were stained with anti-Cyto c (red).
(C) Baxβ potently induces Cyto c release from isolated mitochondria. Mitochondria isolated from HCT116-Bax+/- cells were incubated with in vitro translated (IVT) proteins. The mitochondria were pelleted by centrifugation. The supernatants (Sup) and mitochondrial pellets were probed with Cyto c or HSP60 antibody.
Published in Molecular Cell. 2009. Jan 15, 33: 15-29
Their work has already been cited in a review by Kim and others (Kim et al., Deadly Splicing: Bax Becomes Almighty. Mol Cell 33, Jan 30, 2009):
“Activation of Bax and Bak by BH3-only molecules triggers mitochondrial apoptosis. In a recent issue of Molecular Cell, Fu et al. (2009) identify a constitutively active isoform of Bax, Baxb, whose activity is tightly controlled by the ubiquitin-proteasome system.”
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