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  current news   Press   selected story    
     
  23 December 2014  
  Systematic Study of Drosophila MicroRNA Functions Using a Collection of Targeted Knockout Mutations
 
 




Authors
Ya-Wen Chen1, Shilin Song1, Ruifen Weng1, Pushpa Verma1, Jan-Michael Kugler1,4, Marita Buescher1,5, Sigrid Rouam3, and Stephen M. Cohen1,2,4,*

1  Institute of Molecular and Cell Biology, 61 Biopolis Drive, Singapore 138673, Singapore
2  Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore
3  Procter and Gamble International Operations SA Singapore Branch, Quantitative Sciences, Statistics    Asia, 70 Biopolis Street, Singapore 138547, Singapore
4  Present address: Department of Cellular and Molecular Medicine, University of Copenhagen, 2200N    Copenhagen, Denmark
5  Present address: Blumenbach Institute of Zoology, Georg-August University of Gottingen, 37077    Gottingen, Germany

*Correspondence: scohen@sund.ku.dk

Published in Developmental Cell on 22 December 2014.

Abstract
MicroRNAs are abundant in animal genomes, yet little is known about their functions in vivo. Here, we report the production of 80 new Drosophila miRNA mutants by targeted homologous recombination. These mutants remove 104 miRNAs. Together with 15 previously reported mutants, this collection includes 95 mutants deleting 130 miRNAs. Collectively, these genes produce over 99% of all Drosophila miRNAs, measured by miRNA sequence reads. We present a survey of developmental and adult miRNA phenotypes. Over 80% of the mutants showed at least one phenotype using a p < 0.01 significance threshold. We observed a significant correlation between miRNA abundance and phenotypes related to survival and lifespan, but not to most other phenotypes. miRNA cluster mutants were no more likely than single miRNA mutants to produce significant phenotypes. This mutant collection will provide a resource for future analysis of the biological roles of Drosophila miRNAs.

Figure Legend: Systematic Survey of miRNA Mutant Phenotypes
Results for the assays scored as continuous quantitative variables are presented by p value. Red indicates p < 0.01; blue indicates p > 0.01.

For more information on Stephen COHEN's laboratory, please click here.