Ruifen Weng1 and Stephen M Cohen1,2
1 Institute of Molecular and Cell Biology
2 University of Copenhagen
Published online ahead of print in Development on 22 September 2015.
Post-transcriptional regulation of stem cell self-renewal by microRNAs is emerging as an important mechanism controlling tissue homeostasis. In this report, we provide evidence that the bantam microRNA controls neuroblast number and proliferation in the Drosophila central brain. bantam also supports proliferation of the transit-amplifying intermediate neural progenitor cells in type II neuroblast lineages. The stem cell factors brat, prospero are identified as bantam targets acting on different aspects of these processes. Thus bantam appears to act in multiple regulatory steps in the maintenance and proliferation of neuroblasts and their progeny to regulate growth of the central brain.
Bantam expression in Drosophila larval neural stem cell lineage.
Upper panel: A single type II NB lineage showing bantam GFP sensor expression (green) in a WT control brain. The type II lineage was labeled by worniu-Gal4, asense-Gal80-driven UAS-RFP (red). bantam sensor GFP (green) was very low in the Dpn+ (grey)/Ase- (blue) type II NB (arrow).
Lower panel: bantam GFP sensor expression in a bantam mutant brain. Note the higher level of sensor (green) in the neuroblast and INPs due to loss of bantam-mediated repression of GFP expression.
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