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  current news   Press   selected story    
     
  17th August 2011  
 

Zebrafish transgenic Enhancer TRAP line database ZETRAP 2.0.

 
 




Members of the project:
Igor KONDRYCHYN, Cathleen TEH, Marta GARCIA-LECEA, Youxin GUAN, Ann KANG and Vladimir KORZH.

Abstract
Since 2006, the IMCB server hosts one of the first databases of transgenic zebrafish strains widely used as living tools for research. This is the Zebrafish Enhancer TRAP lines database (ZETRAP). It promotes dissemination of information about the developmental and genomic aspects of 27 transgenic zebrafish lines. These lines express the cytosolic version of EGFP and were obtained using Tol2 transposon-mediated transgenesis during a primary screen back in 2002. Some of these lines have been transferred to more than 30 laboratories in 12 countries and have been used in many publications. Furthermore, several hundred transgenics have been generated by a systematic re-jump of a transposon from the two distinct genomic sites in transgenics from a first screen (Kondrychyn et al., 2009). This collection has since been expanded by a generation of transgenics expressing the membrane-tethered version of a novel photodynamic protein, KillerRed (Teh et al., 2010). The genomic and expression pattern information collected as a result of systematic investigation of some of these lines was compiled in an updated version of this database - ZETRAP 2.0, which contains the sequence of regions flanking the insertion sites, links to genes in zebrafish genome and confocal images of 124 transgenics. ZETRAP 2.0 went online in early August 2011 and is accessible here: http://plover.imcb.a-star.edu.sg/webpages/home.html .

This updated database would not have been possible without the efforts of a trainee student in the lab. Special thanks to Ms. Youxin Guan from Victoria Junior College who transferred the data generated by VK´s lab during the last five years into this database.

 
 

 
 


Figure Legend: Unlike ZETRAP, the new version of the database, ZETRAP 2.0, includes transgenics expressing not only cytosolic GFP (ET33-E20) but also the membrane-tethered red fluorescent protein KillerRed. The KR19 is a transgenic line shows a very similar expression pattern, and provides us with the possibility to observe the differential distribution of proteins in the choroid plexus cells of compound transgenics (ET33-E20 x KR19) expressing both proteins.

For more information on Vladimir KORZH’s laboratory, please click here.