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  current news   Press   selected story    
     
  16th March 2011  
 

Drosophila miR-14 regulates insulin production and metabolism through its target sugarbabe

 
 




Authors
Jishy VARGHESE, Singfee LIM and Stephen COHEN.

Institute of Molecular and Cell Biology, 61 Biopolis Drive, Singapore 138673.

Published in Genes & Dev. 24: 2748-2753 (2010).

Abstract
Energy homeostasis depends on insulin signaling in metazoans. Insulin levels reflect the nutritional status of the animal to control levels of circulating sugar and to regulate storage of resources in the form of glycogen and fat. Over the past several years evidence has begun to accumulate that insulin production and secretion as well as cellular responsiveness to insulin are subject to regulation by microRNAs. Here we present evidence that miR-14 acts in the insulin producing neurosecretory cells in the adult Drosophila brain to control metabolism. miR-14 acts in these cells through its direct target sugarbabe. sugarbabe encodes a predicted zinc finger protein that regulates insulin gene expression in the neurosecretory cells. Regulation of sugarbabe levels by nutrients and by miR-14 combines to allow the fly to manage resource mobilization in a nutritionally variable environment.

 
 

 
 


Figure Legend : sugarbabe expression is specific to insulin producing neurosecretory cells (IPCs) and the regulation of sugarbabe by miR-14 is important for regulating body fat levels. (A) sugarbabe expression in the adult IPCs using fluorescent in situ hybridization (green). IPC marked by dILP2-Gal4 nRFP (red). (B) sugarbabe mRNA levels from expression profiling data. Comparing control, miR-14 mutant, and rescue - miR-14 mutant, dilp2-Gal4 UAS-miR-14 conditions. (C) Partial suppression of miR-14 mutant phenotype by IPC-specific depletion of sugarbabe. Comparing control to miR-14 mutant, UAS-sug-RNAi (without the Gal4 driver, middle)and miR-14 mutant, dilp2-Gal4 UAS-sug-RNAi (with the Gal4 driver).

This work was also selected for the cover of the issue. Please click here to view their feature.

For more information on Stephen COHENís laboratory, please click here.