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  current news   Press   selected story    
     
  15 February 2017  
 
Mechanisms of Yersinia YopO kinase substrate specificity
 
 




Authors
Wei Lin Lee, Pavithra Singaravelu, Sheena Wee, Bo Xue, Khay Chun Ang, Jayantha Gunaratne, Jonathan M. Grimes, Kunchithapadam Swaminathan & Robert C. Robinson.

Wei Lin LEE, Pavithra SINGARAVELU and Sheena WEE contributed equally to this work.

Published online Scientific Reports on 4 January 2017.
Please see http://www.nature.com/articles/srep39998

Abstract
Yersinia bacteria cause a range of human diseases, including yersiniosis, Far East scarlet-like fever and the plague. Yersiniae modulate and evade host immune defences through injection of Yersinia outer proteins (Yops) into phagocytic cells. One of the Yops, YopO (also known as YpkA) obstructs phagocytosis through disrupting actin filament regulation processes - inhibiting polymerization-promoting signaling through sequestration of Rac/Rho family GTPases and by using monomeric actin as bait to recruit and phosphorylate host actin-regulating proteins. Here we set out to identify mechanisms of specificity in protein phosphorylation by YopO that would clarify its effects on cytoskeleton disruption. We report the MgADP structure of Yersinia enterocolitica YopO in complex with actin, which reveals its active site architecture. Using a proteome-wide kinase-interacting substrate screening (KISS) method, we identified that YopO phosphorylates a wide range of actin-modulating proteins and located their phosphorylation sites by mass spectrometry. Using artificial substrates we clarified YopO’s substrate length requirements and its phosphorylation consensus sequence. These findings provide fresh insight into the mechanism of the YopO kinase and demonstrate that YopO executes a specific strategy targeting actin-modulating proteins, across multiple functionalities, to compete for control of their native phospho-signaling, thus hampering the cytoskeletal processes required for macrophage phagocytosis.

Figure 1



Figure Legend: Crystal structure of YopO-actin:MgADP.

(a) Crystal structure of the kinase (light blue) and GDI (pale yellow) domains in complex with actin (pale green). MgADP lies in the active site of the YopO kinase domain. (b) Structure of the kinase domain of YopO. Regions that are ordered in this ADP-bound structure, but were disordered in the apo structure (PDB: 4CI6), are colored pink and labeled I–IV with reference to Supplementary Fig. 1.

For more information on Robert C. ROBINSON's lab, please click here.