News archives


OCTOBER - DECEMBER 17

JULY - SEPTEMBER 17

APRIL - JUNE 17

JANUARY - MARCH 17

OCTOBER - DECEMBER 16

JULY - SEPTEMBER 16

APRIL - JUNE 16

JANUARY - MARCH 16

OCTOBER - DECEMBER 15

JULY - SEPTEMBER 15

APRIL - JUNE 15

JANUARY - MARCH 15

OCTOBER - DECEMBER 14

JULY - SEPTEMBER 14

APRIL - JUNE 14

JANUARY - MARCH 14

OCTOBER - DECEMBER 13

JULY - SEPTEMBER 13

APRIL - JUNE 13

JANUARY - MARCH 13

OCTOBER - DECEMBER 12

JULY - SEPTEMBER 12

APRIL - JUNE 12

JANUARY - MARCH 12

OCTOBER - DECEMBER 11

JULY - SEPTEMBER 11

APRIL - JUNE 11

JANUARY - MARCH 11

OCTOBER - DECEMBER 10

JULY - SEPTEMBER 10

APRIL - JUNE 10

JANUARY - MARCH 10

OCTOBER - DECEMBER 09

JULY - SEPTEMBER 09

APRIL - JUNE 09

JANUARY - MARCH 09

OCTOBER - DECEMBER 08

JULY - SEPTEMBER 08

APRIL - JUNE 08

JANUARY - MARCH 08

OCTOBER - DECEMBER 07

JULY - SEPTEMBER 07

APRIL - JUNE 07

JANUARY - MARCH 07

 
  current news   Press   selected story    
     
  14th November 2013  
  Single-Cell Profiling of Epigenetic Modifiers Identifies PRDM14 as an Inducer of Cell Fate in the Mammalian Embryo
 
 




Authors
Adam Burton1,6, Julius Muller2,6, Shengjiang Tu4, Pablo Padilla-Longoria3, Ernesto Guccione2,5,* and Maria-Elena Torres-Padilla1,*

1 Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS/INSERM U964, Universite de     Strasbourg, F-67404 Illkirch, CU de Strasbourg, France
2  Division of Cancer Genetics and Therapeutics, Laboratory of Chromatin, Epigenetics and     Differentiation, Institute of Molecular and Cell Biology, Agency for Science, Technology and Research,     Singapore 138673, Singapore
3  Instituto de Investigaciones en Matematicas Aplicadas y en Sistemas, Universidad Nacional Autonoma     de Mexico, C.U., Distrito Federal 04510, Mexico
4  Howard Hughes Medical Institute, Department of Biochemistry, New York University School of     Medicine, 522 First Avenue, New York, NY 10016, USA
5 Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore,     Singapore 119228, Singapore
6  These authors contributed equally to this work

*Corresponding authors

Published in Cell Reports on 31 October 2013.

Abstract

Cell plasticity or potency is necessary for the formation of multiple cell types. The mechanisms underlying this plasticity are largely unknown. Preimplantation mouse embryos undergo drastic changes in cellular potency, starting with the totipotent zygote through to the formation of the pluripotent inner cell mass (ICM) and differentiated trophectoderm in the blastocyst. Here, we set out to identify and functionally characterize chromatin modifiers that define the transitions of potency and cell fate in the mouse embryo. Using a quantitative microfluidics approach in single cells, we show that developmental transitions are marked by distinctive combinatorial profiles of epigenetic modifiers. Pluripotent cells of the ICM are distinct from their differentiated trophectoderm counterparts. We show that PRDM14 is heterogeneously expressed in 4-cell-stage embryos. Forced expression of PRDM14 at the 2-cell stage leads to increased H3R26me2 and can induce a pluripotent ICM fate. Our results shed light on the epigenetic networks that govern cellular potency and identity in vivo.

Figure Legend: Expression levels of 35 chromatin modifiers in single cells derived from embryos at all stages, from oocyte to blastocyst, were subjected to hierarchical clustering. Important clusters and trends were identified and visualized by Principal Component Analysis (PCA) and a semiquantitative epigenetic landscape was computed from the mean values (centers of gravity) and SDs (spread) of the individual cells for each developmental stage in the PCA.

To see video abstract, please click here.

For more information on Ernesto GUCCIONE's laboratory, please click here.