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  current news   Press   selected story    
     
  14 September 2012  
  Cis-2-dodecenoic acid receptor RpfR links quorum-sensing signal perception with regulation of virulence through cyclic dimeric guanosine monophosphate turnover
 
 



Authors
Yinyue Denga,1,2, Nadine Schmidb,1, Chao Wanga, Jianhe Wanga, Gabriella Pessib, Donghui Wua, Jasmine Leea, Claudio Aguilarb, Christian H. Ahrensc, Changqing Changa, Haiwei Songa, Leo Eberlb,2, and Lian-Hui Zhanga,2.

a- Institute of Molecular and Cell Biology (IMCB), A*STAR (Agency for Science, Technology and     Research), Singapore
b- Department of Microbiology, University of Zürich, CH-8008 Zürich, Switzerland
c- Institute of Molecular Life Sciences, University of Zürich, CH-8057 Zürich, Switzerland
1- Y.D. and N.S. have contributed equally to this work.
2- Corresponding authors

Published ahead of print in PNAS on 4th September 2012.

Abstract
Many bacterial pathogens produce diffusible signal factor (DSF)-type quorum sensing (QS) signals in modulation of virulence and biofilm formation. Previous work on Xanthomonas campestris showed that the RpfC/RpfG two-component system is involved in sensing and responding to DSF signals, but little is known in other microorganisms. Here we show that in Burkholderia cenocepacia the DSF-family signal cis-2-dodecenoic acid (BDSF) negatively controls the intracellular cyclic dimeric guanosine monophosphate (c-di-GMP) level through a receptor protein RpfR, which contains Per/Arnt/Sim (PAS)-GGDEF-EAL domains. RpfR regulates the same phenotypes as BDSF including swarmingmotility, biofilm formation, and virulence. In addition, the BDSF− mutant phenotypes could be rescued by in trans expression of RpfR, or its EAL domain that functions as a c-di-GMP phosphodiesterase. BDSF is shown to bind to the PAS domain of RpfR with high affinity and stimulates its phosphodiesterase activity through induction of allosteric conformational changes. Our work presents a unique and widely conserved DSF-family signal receptor that directly links the signal perception to c-di-GMP turnover in regulation of bacterial physiology.

Figure Legend: ITC analysis of the molecular interaction between BDSF and RpfR. (A) ITC titration of 20 μM RpfR with 200 μM BDSF in PBS buffer at 21 °C. (B) ITC titration of 20 μM RpfR with 200 μM of the saturated isomer of BDSF in PBS buffer at 21 °C.

For more information on Lianhui ZHANG’s laboratory, please click here.