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  current news   Press   selected story    
     
  7 November 2014  
  Mastl is required for timely activation of APC/C in meiosis I and Cdk1 reactivation in meiosis II
 
 




Authors
Deepak Adhikari1, M. Kasim Diril2, Kiran Busayavalasa1, Sanjiv Risal1, Shoma Nakagawa3, Rebecca Lindkvist1, Yan Shen1, Vincenzo Coppola4, Lino Tessarollo4, Nobuaki R. Kudo3, Philipp Kaldis2,5,*, and Kui Liu1,*

*  corresponding authors

1  Department of Chemistry and Molecular Biology, University of Gothenburg, S-405 30 Gothenburg,    Sweden
2  Institute of Molecular and Cell Biology, A*STAR, Singapore 138673, Republic of Singapore
3  Institute of Reproductive and Developmental Biology, Department of Surgery and Cancer,    Hammersmith Hospital, Imperial College London, London W12 0NN, England, UK
4  National Cancer Institute, Mouse Cancer Genetics Program, National Cancer Institute–Frederick,    Frederick, MD 21702
5  Department of Biochemistry, National University of Singapore, Singapore 117599, Republic of    Singapore

Published in Journal of Cell Biology, 206, 843-853 on 22 September 2014.

This article has been recommended in F1000Prime as being of special significance in its field. Please see http://f1000.com/prime/718885762?ref=ypp

Abstract

In mitosis, the Greatwall kinase (called microtubuleassociated serine/threonine kinase like [Mastl] in mammals) is essential for prometaphase entry or progression by suppressing protein phosphatase 2A (PP2A) activity. PP2A suppression in turn leads to high levels of Cdk1 substrate phosphorylation. We have used a mouse model with an oocyte-specific deletion of Mastl to show that Mastl-null oocytes resume meiosis I and reach metaphase I normally but that the onset and completion of anaphase I are delayed. Moreover, after the completion of meiosis I, Mastl-null oocytes failed to enter meiosis II (MII) because they reassembled a nuclear structure containing decondensed chromatin. Our results show that Mastl is required for the timely activation of anaphase-promoting complex/cyclosome to allow meiosis I exit and for the rapid rise of Cdk1 activity that is needed for the entry into MII in mouse oocytes.

Figure Legend: Meiotic maturation of OoMastl-/-oocytes.
Representative images of immunostaining for DNA, CREST, and spindle showing normal progression to metaphase I in OoMastl-/- oocytes. Oocytes were cultured for the indicated periods after GVBD and were fixed. 30 oocytes were analyzed for each time point.

For more information about the laboratory of Philipp KALDIS, please click here.