Huashan Wang1,2, Yu Cai3, 4, William Chia3, 5 and Xiaohang Yang1, 2.*
1 Drosophila Neurobiology Lab, Institute of Molecular and Cell Biology, Proteos Building, Singapore; 2 Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Singapore;
3 Temasek Lifesciences Laboratory, National University of Singapore, Singapore; 4 Department of Biochemistry, National University of
Singapore, Singapore and 5 Department of Biological Sciences, National University of Singapore, Singapore
During neuroblast divisions, cell fate determinants Prospero and Numb, together with their adaptor proteins Miranda and Pon, localize to the basal cell cortex at metaphase and segregate exclusively to the future ganglion mother cells at telophase. In inscuteable mutant neuroblasts, these basal proteins are mislocalized during metaphase. However, during anaphase/telophase these mutant neuroblasts can partially correct these earlier localization defects and redistribute cell fate determinants as crescents to the region where the future ganglion mother cell “buds” off. This compensatory mechanism has been referred to as “telophase rescue”. We demonstrate that DTRAF1, the Drosophila homolog of the mammalian tumor-necrosis factor receptor-associated factor, and Eiger, the homolog of the mammalian tumor-necrosis factor, are required for telophase rescue of Miranda/Prospero. DTRAF1 localizes as an apical crescent in metaphase neuroblasts and this apical localization requires Bazooka and Eiger. The Miranda/Prospero telophase rescue seen in inscuteable mutant neuroblasts requires DTRAF1. Our data suggest that DTRAF1 binds to Bazooka and acts downstream of Eiger in the Miranda/Prospero telophase rescue pathway.
Published online in 30 November 2006 on The EMBO Journal (2006) 25, 5783–5793.
Drosophila homologs of mammalian TNF/TNFRrelated molecules regulate segregation of Miranda/Prospero in neuroblasts (PDF).
Please click here to download. .