Figure Legend: dmOCA-S is recruited to Drosophila Histone Locus Bodies during S phase. (A) A transcriptional regulation pathway of D. melanogaster core histone genes. All the core histone genes contain multiple octamer sites in their promoters for recruitment of the common transcription factor Pdm-1, which in turn recruits dmOCA-S that might well be the universal co-activator for the coordinated expression of all Drosophila core histone genes. (B-E) Random cells images. (F) Cell cycle profiles of random cells; ~20% of the cells were in the S-phase. A similar percentage was obtained when counting 100 randomly picked cells using HLB-foci-staining as a criterion. (G-J) Early S-phase cells images that show weak HLB foci and co-localization with nuclear dmGapdh. (K) Early S-phase cell cycle profiles. (L-O) Mid-S-phase cells images that show prominent HLB foci with strong co-localization with nuclear dmGapdh foci. (P) Mid-S-phase cell cycle profiles. (Q-T) Late S- and early G2-phase cells images that show decreased nuclear dmGapdh and HLB foci in size and number. (U) Late S- and early G2-phase cell cycle profiles. (V-Y) G2-phase cells, with no HLB and dmGapdh nuclear foci. (Z) G2-phase cell cycle profiles. When appropriate, arrows indicate HLB (B, G, L, Q, V) and nuclear dmGapdh (C, H, M, R, W) foci and their nuclear co-localization (D, I, N, S, X), which was confirmed by superimposing to DAPI nuclei-staining images (E, J, O, T, Y). HLB foci were stained with the MPM-2 antibody; nuclear dmGapdh foci were stained by anti-p38/GAPDH antibodies. Bar, 10 μM.
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