Terk Shin Teng, Baohong Lin, Ed Manser, Dominic Chi Hiung Ng and Xinmin Cao.
Figure Legend: Loss of Stat3 expression affects directional migration. (A) ΔSt3 MEF exhibited altered morphology during migration. Phase-contrast images of WT and ΔSt3 MEFs at 0 and 24 hours after wounding. (B) Staining of the actin cytoskeleton in migrating cells. Confluent monolayer of WT and ΔSt3 MEFs were wounded and then fixed and stained with DAPI and Alexa Fluor 488-conjugated phalloidin after 5 hours. (C) ΔSt3 MEFs exhibited a non-polarized phenotype during migration. Phase-contrast images of WT and DSt3 MEFs collected during random migration. (D) Analysis of migration rate and directional persistence by random migration assay. WT and DΔSt3 cells were plated sparsely and monitored by time-lapse microscopy for 16 hours. Rate and persistence of migration were quantified. (E) Migration tracks of WT and ΔSt3 MEFs. Tracks from 20 random cells from (D) were plotted using Excel.
published in Journal of Cell Science, Nov. 15, 2009, Vol 122, Issue 22.
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